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PPM
(Plant Preservative Mixture)
PPM is the ultimate
solution to the ongoing struggle against microbial airborne, waterborne and endogenous
contamination.
PPM is a broad-spectrum
preservative/biocide, which effectively prevents or reduces microbial contamination in
plant tissue culture.
At optimum doses, PPM
is an extremely effective preservative/biocide, yet does not impair in vitro germination,
callus proliferation and callus regeneration.
PPM is used
successfully by well-known research institutions, universities, commercial companies,
private laboratories, and nurseries worldwide, in a wide variety of applications.
NOTE:
PPM should be used by individuals highly trained and experienced in safety procedures and
proper lab techniques. It is recommended to review the MSDS prior to utilizing PPM.
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Compton, Michael E. and Jennifer M. Koch. 1999. Influence of Plant
Preservative Mixture (PPM) on adventitous organogenesis. In Vitro - Plant 35(3): 40-A.
Compton
Michael E. and Jenniffer M. Koch 2001. Influence of Plant Preservative Mixture ( PPM ) on
adventitious organogenesis in melon, petunia and tobacco. In Vitro Cell Dev. Biol.- Plant
37: 259-261.
Guri,
Assaf Z. and Kishor N. Patel. 1998. Compositions and methods to prevent microbial
contamination of plant tissue culture media. United States Patent 5,750,402 (Go to: and
type in "Guri" in the search box in the US patent office patent website).
Guri, Assaf Z. 1998. Advances in Controlling Plant Tissue Culture Contamination Using the
New Biocide PPM (Poster Presentation). XXXVII Congresso Viterbo 28-30 Septembre 1998,
Societa Italiana di Fisiologia Vegetale.
Lunghusen, John. 1998. An effective biocide for plant tissue culture. Australian
Horticulture (January 1998): 46-48.
M.P. Fuller and T. Pizzey., Vitro Cult. & Hort. Breeding, Eds. S. Sorvari
et al. Acta Hort 560, ISHS 2001 "Teaching Fast and Reliable Plant Tissue Culture
Using PPM and Brassicas".
M. Babaoglu & M. Yorgancilar, Plant Cell. Tissue. Organ.
Culture 2000 440 : 31-34.
Niedz, Randall P. 1998. Using isothiazolone biocides to control microbial and fungal
contaminants in plant tissue cultures. HortTechnology 8 (4): 598-601.
Renfroe, M.H., J.T. Hitt, J.P. McNicholas, J.A. Priday and V.B. Delgaiza. 1999. Comparison
of laboratory and field Portulaca culture establishment techniques. In Vitro - Plant
35(3): 53-A.
Sandra M. Reed J. Environ., Hort. 2000 18(1) 34-39
Stiff, Carol M. 1998. Plant tissue culture for the classroom and home: A manual to
accompany the Kitchen Culture Kit. Kitchen Culture Kits, Inc. 72 p.
Stiff, Carol M. 1999. Successful plant tissue culture in the classroom or home. In Vitro -
Plant 35(3): 53-A.
Stiff, Carol M. 1999. Plant tissue culture for the classroom and home: An interactive CD
with lessons, lectures, labs, and technical manual. Kitchen Culture Kits, Inc.
www.kitchenculturekit.com |