Plant Tissue Culture in the Classroom and Home
for the Serious Teacher, Gardener, or Hobbyist
Plant tissue culture involves the sterile
growth of plants in bottles for the purpose of mass production.
Through the use of plant hormones and other
growth regulators, small plant parts can be induced to produce hundreds of small
"plantlets" which can be further developed and grown in greenhouses or as house
plants.
Using a microwave oven or a pressure cooker,
supplies found in your kitchen, plus the contents of the newly developed "Kitchen
Culture Kit©, you can mass propagate hundreds of your favorite plants in your
kitchen or classroom.
In this workshop you will make your own
media, disinfect and culture plant leaves, axillary buds and seeds, and discuss trouble
shooting and internet resources.
| Time |
Tentative Schedule (Photos) for Beginner Class |
| 9:00 |
Introductions
Fill out information sheets; hand out class schedule
Discuss what everyone wants to accomplish
Safety and food; location of aprons, gloves, goggles, etc. |
| 9:20 |
Presentation
on plant tissue culture in the home and classroom (part 1) |
| |
Break |
| 10:20 |
Media
preparation using a microwave: demo by instructor followed by media preparation by
students (break into groups) |
| 11:20 |
Discuss
clean boxes and aseptic technique
Assemble PVC boxes and prepare areas |
| 12
Noon |
Lunch
(provided) and discussion |
| 1:00 |
Demo
of disinfection and culture of: leaf, axillary bud, seeds |
| 1:15 |
Students disinfect and culture:
--African violet leaves
--Axillary buds
--Seed or orchid pod (if available)
--Other? |
| |
Break
(as it fits your individual schedule) |
| 4:00 |
Discuss
problems, trouble shooting, alternative protocols, internet connection and webpage
resources;
Share experiences with "seasoned tissue culturists" at meeting |
| 5:00 |
Workshop
officially ends/discussions continue as needed |
| |
|
| |
Tentative Schedule for Advanced
Class
(Developed at request of specific groups)
Contact Carol with your requests. |
| 9:00 |
Introductions/background
of experience/plants of interest |
| 9:30 |
Advanced
techniques for media preparation:
Dependent on student requests |
| 10:30 |
Demonstrations
of specific dissection of dicots and monocots of interest using video projection
equipment
Dependent on student requests |
| 12
Noon |
Lunch/discussion |
| 1
- 5 p.m. |
Advising
on specific plants; assistance from local experts when available |
|
Registration
|
Cost
= $65 - 130/person
( Cost varies with distance travelled [drive vs. fly]. Discounts are available to
schools, gardening groups, others; contact Carol Stiff at kck@turbonet.com )
Note: some workshops are offered through other groups or schools and payment is made
directly to them. Others are organzied through KCK and payment is made directly to
KCK. Email Carol at kck@turbonet.com for
further information.
- Special Rate for Kit + DVD = $100 for workshop participants
if ordered before the workshop. See registration form.
|
Email us to reserve a place in class
Registration can be paid online via
credit card once we determine there is a sufficient number of students: www.kitchenculturekit.com/catalog.htm
Alternate payment methods are
possible. Email or call us for options. Contact
Carol at kck@turbonet.com
- Workshop is subject to cancellation if insufficient
enrollment or weather related problems.
|
We are looking for site coordinators in all regions of the US.
Please contact us if you are interested.
A site
coordinator:
must be a KCK customer or be
experienced in plant tissue culture or willing to learn
prepares media and sterile water for
the class (Baby food jars, Phytocaps, media, etc. will be shipped to the site
coordinator.) or helps instructor prepare these things the night before the workshop
helps arrange classroom rental or
location (free rent is preferred)
- -- classroom should be WITHOUT CARPET
- -- have a water source in the building
- -- have long tables (29" x 72 " or 29"
x 48") or lab benches
- -- chairs or stools for 20 people
- -- electricity
- -- empty wall or projector screen (to project
presentation)
purchases isopropyl alcohol, bleach,
and other items that do not "fly" well
provides transportation for the
instructor from hotel to classroom
arranges coffee break beverages and
snacks and delivery of lunch.
will be reimbursed
for all requested expenses
- gets free registration for the workshop
gets a free kit and DVD or
equilvalent in supplies
does not get rich but gets to meet
nice people at the workshop
NOTE: many of these responsibilities
are not applicable if the instructor drives to the workshop site (rather than fly) and
brings the required items in the car
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